Applied for testing the intragroup differences between several related samples PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20460822 and Wilcoxon test was used PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/14960617 when Friedman test was significant, to assess within group changes from baseline to the subsequent time points. A trend analysis in quartiles of variables was performed by the chi square test to estimate the cut off levels in relation to LV thrombus. Logistic regression analyses with LV thrombus as the dependent variable were performed with adjustments for infarct size. Correlation 3-Amino-1H-indazole-4-carbonitrile analysis was done with Spearmans rho. All tests were two-sided, and p values <0.05 were considered statistically significant. PASW software package version 18.0 for Mac OS X was used for data analyses.The TF mRNA expressions were numerically higher in the LV thrombus group within the first 6? days after the AMI, however, not statistically significant (p>0.3 for all time points) (Figure 2). At later time points no between group differences were detected in the mRNA levels of TF.Prothrombin fragment 1+A different pattern could be demonstrated for F1+2, with significantly lower levels in the thrombus group after 2? weeks and 3 months (p<0.01) (Figure 1, Panel B). In both groups, the F1+2 levels declined from 4? days to later time points.Endogenous thrombin potentialA similar pattern as for F1+2 was found for ETP, with significantly lower levels in the thrombus group 8? days,Table 1 General characteristics of the patients with and without LV thrombus formationVariable No (n=85) 57(50,64) 15(18 ) 37(44 ) 27(32 ) 39(46 ) 6(7 ) 4(5 ) 84.0 (74.5,92.5) 130 (120,144) Yes (n=15) 63(50,69) 1(7 ) 7(47 ) 7(47 ) 9(60 ) 2(13 ) 0(0 ) 80 (77.0,92.0) 135 (130,150) p-value 0.18 0.49 0.20 0.41 0.47 0.76 0.89 0.99 0.33 <0.01 <0.ResultsGeneral characteristicsAge (years) Female Current smoker Hypertension Hyperlipidemia Diabetes mellitus Previous myocardial infarction Weight (kg) Systolic blood pressure (mmHg) Peak CK (ugr/L) Infarct size ( of LAD area by SPECT)Primary PCI was performed in 71 patients, facilitated PCI in 15 patients and rescue PCI in 14 patients without any differences between the groups. As previously described, we detected LV thrombus formation in 15 of 100 patients within the first 3 months, most of them diagnosed during the first week after the AMI [5]. LV thrombus formation was diagnosed by echocardiography in 13 patients and by MRI in 2 patients. No between group differences in baseline characteristics was found, except from significantly higher peak CK levels and larger infarct size assessed by SPECT in the patients with LV thrombus formation (Table 1).Prothrombotic markers Tissue factor2197 6128 (1236,4182) (4556,8061) 63.8 (40.5,76.8) 82.5 (71.3,93.1)The levels of TF were significantly higher in the LV thrombus group 4? days, 8? days and 3 months after the AMI compared to the patients without thrombus formation (Figure 1, Panel A). In both groups, the TF levelsHypertension is defined as antihypertensive treatment or measured blood pressure above 140/90 mmHg. Hyperlipidemia is defined as baseline fasting total cholesterol above 5.5 mmol/L or previous use of cholesterol lowering medication. P-values refer to differences between the LV 3-Nitro-6-(trifluoromethyl)pyridin-2(1H)-one thrombus group and those without thrombus formation. CK; creatine kinase, LAD; the left anterior descending artery, SPECT; single photon emission computer tomography.Solheim et al. Thrombosis Journal 2013, 11:1 http://www.thrombosisjournal.com/content/11/1/Page 4 ofFigure 1 Circulating levels of tissue factor, F1+2, D-dimer.